By Morris J., Lycett J.
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Extra info for A Monograph of the Mollusca from the Great Oolite, Chiefly from Minchinhampton and the Coast of Yorkshire, Part I: Univalves
McCreery, L. Blank, and M. Karolczak, Eur. J. Pharmacol. 17, 287 (1972). 10 R. F. Chapman, A. Percival, and G. A. Swan, J. Chem. , C, p. 1664 (1970). 11 S. Senoh, and B. Witkop, J. Am. Chem. Soc. 81, 6231 (1959). lz F. -Y. Bae, A. R. Jacobson, Y. Lee, and L. M. Sayre, J. Org. Chem. 59, 2409 (1994). 13 F. G. H. Lee, D. E. Dickson, and A. A. Manian, J. Med. Chem. 14, 266 (1971). [51 COPPER AMINE OXIDASES 55 TABLE I CATALYTIC AEROBIC DEAMINATIONSMEDIATED BY TPQ MORSEL2" Substrate pH Buffer, conc. 0 Phosphate, 5 mM c mM c Time (hr) Yield" (%) 5 18 4 23 4 23 3 10 3 4 9 23 48 4 23 4 23 14 4 23 4 23 250 294 290 420 180 240 330 410 350 385 470 610 630 30 39 86 99 180 37 146 53 147 The reactions are 50 m M in amine and 1 mM in 2, conducted at 26° in buffer-CH3CN (7 : 3).
The preparation of active-site peptides o f derivatized e n z y m e s is discussed elsewhere (see , this volume). Figure 1C illustrates the absorbance spectra of the p-nitrophenylhydrazine derivative of a m o d e l topa aquinone hydantoin, 2 with absorbance maxima at 455 n m in neutral solution and 575 n m in 1 M K O H .  SPECTROPHOTOMETRIC DETECTION OF TOPA QUINONE 0~ H,,,,'N~' -0 v 37 N , ~ -NO2 NO2 FIG. 2. Proposed structures for the p-nitrophenylhydrazine derivative of topa aquinone at p H 7 (left) and pH 14 (right).
M. Dooley, Biochem. J. 288, 337 (1992).  33 TOPA QUINONE IN COPPER AMINE OXIDASES TABLE IV SUMMARYFOR PHENYLHYDRAZINE, THERMOLYSINPROTOCOL Copper amine oxidase Phenylhydrazine bound (%) ~max of enzymatic phenylhydrazone (nm) TPQ-peptide yield (%) Bovine serum Porcine plasma Pig kidney Pea seedling 87 59 38 100 448 450 443 443 34 32 15 26 sequencing, mass spectral analysis, UV/VIS spectrophotometry, and resonance Raman spectra to establish the presence of TPQ in other copper amine oxidase peptides.
A Monograph of the Mollusca from the Great Oolite, Chiefly from Minchinhampton and the Coast of Yorkshire, Part I: Univalves by Morris J., Lycett J.