By P.G. LeFevre
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Additional resources for Active Transport through Animal Cell Membranes
The kidneyslice experiments discussed above are especially subject to such interpretation. BARTLEY and DAVIES '(1952), analysing sheep kidney-cortex mitochondria after incubation at 20° C. 1. They noted further (BARTLEY and DAVIES 1954) that these preparations maintained large K+ and Na+ concentration ratios for hours at 0° C. in a deficient medium (so that the absolute amounts were small); but that at 20° C. the extra NaT and K + were lost rapidly unless the ingredients mentioned above were available.
And THAN HAUSER 1949). In these experiments, if a complete medium was presented, a strong preferential uptake of K + was apparent; but, in the absence of Mg++, Na+ was distinctly preferred. The presence of K + augmented the rate of phosphate incorporation. ROBERTS and ROBERTS (1950) found similarly that supplying K+ to K+depleted E. coli greatly enhanced their incorporation of P32. Analysis showed a fairly linear relation between cell [K +I and p32 incorporation, if <19 Transport of Inorganic Anions other limiting conditions were made non-critical.
The K42 exchange rate in this system even at 0° C. was too rapid to be es-timated. The Na 24 turnover at 0° C. indicated that about 20% of the Na+ was exchangeable only with some difficulty, unless substrates such as fumarate or ketoglutarate were available. 41 Transport of Inorganic lations Table IX. Maintenance of Ionic Content of Sheep Kidney-Cortex Particulates in vitro. ) Treatment and Additions Concentration ratios (particles/medium) Na+ K+ Aerobic; no addition pyruvate, 5 mM. . . ATP,lmM .
Active Transport through Animal Cell Membranes by P.G. LeFevre